Tools & Settings

Tools

Toolbar

The toolbar is located on the left side of the main window and gives quick access to a set of functions described below:

Tool	Name	Description
	Scalebar	Toggles the scale bar at the bottom of the sample display
	Navigation tool	Tool to navigate the worktable
	Take screenshot	The screenshot can be exported later in different formats
	Pressure tool	Manual pressure control
	Oscilloscope	Shows signal streams
	Probe tool	Manual z positioning
	Wash tool	Save and run wash cycles
	Accessible Plate Area	Displays bounds of accessible plate area on video feed
	Show Result Data	Shows recorded data (e.g. screenshots)

Navigation tool

Basic functionality

The navigation tool shows the current position of the head on the installed plates. The head position is marked by the green point. By clicking on a well, the CERES controller will move its head to the selected well.

Positioning

Relative

A well wider and higher than 10mm allows relative positioning. Click on any point within the well and the CERES controller will move its head there.

Absolute

A well containing a point pattern can always be approached with absolute positions. Simply click on the well and select the position in the dialog box which just appeared. These wells are marked by a point pattern.

The dialog box allows the user to move to:

• the well’s center
• the point pattern’s center
• any point in the point pattern
• a point relative to the well’s center

If a well does not contain a point pattern and is less wide or less high than 10mm, the CERES controller will always move to the center of the well. These kind of wells show a cross at the center when hovered over with the mouse.

Note

To use custom plates, have a look a the plate editor to create a model for the CAPA Operator Software.

Pressure tool

This tool allows to directly control the pressure applied to the cantilever. The currently active pressure is highlighted in gold.

Adding, editing or deleting custom pressure values can be performed by using the corresponding buttons on the right.

Add

Adds the current target pressure to the list of custom pressure values. The pressure value can be directly changed using the number pad. Custom pressures are maintained between restarts of the controller software.

Delete

Enters deletion mode: When in this mode, clicking on a custom pressure will delete it.

Edit

Enters edit mode: When in this mode, clicking on a custom pressure allows its value to be changed. If an active pressure value is changed, the new value is applied immediately.

Oscilloscope

The oscilloscope icon toggles an animated graph which displays the deflection and the pressure of the last few seconds.

Right next to the graph, the current values of the deflection and the pressure can be seen.

The scaling of the rulers on either side can be adjusted. By default, it will automatically scale to show all recent values. If a fixed total range is desired, the zoom can be extended by expanding the zoom combo box and choosing a value. Additionally, the mouse scroll wheel can be used to scroll through all available zoom levels.

Note that the deflection signal always shows uncorrected values. Therefore, one will observe a corresponding shift of the signal when applying a relative setpoint but the signal itself will not necessarily correspond to that setpoint.

Probe tool

Control the movement of the probe using the following options (left to right):

Approach

Pressing triggers an approach operation to the configured setpoint; pressing the icon again aborts the operation

Move to content height

Pressing triggers a movement to the corresponding plate’s content height. Pressing the icon again aborts the movement.

Home position

Retracts the cantilever to the uppermost position. Pressing the icon again aborts the movement.

Retract

Retracts the probe by the configured distance. Pressing the icon again aborts the movement.

Advance

Moves the cantilever quickly towards the sample; a second click aborts the movement. Due to the high crash risk, the operation must first be unlocked by clicking on the lock.

Wash tool

A wash cycle can be set up for the probe using the wash tool, either in the sample rack or in dedicated wells of a 12 well-plate (Well A1 to C4).

• Fill empty, unused wells with chosen washing solutions
• Define a dipping time and a pressure value
• After the last step, the probe stays in the last container to avoid the drying of the probe

As an example:

1. Bleach in well A2, 5s at 100mbar
2. Water in well B2, 30s at 10mbar
3. Water in well C2, 2s at 0mbar, the probe will stay here as long as it has no new command

Creating a wash sequence

A wash sequence can only be edited if the plate view is visible on the left side of the wash sequence. If the plate view is not visible, click on the Edit button.

• To add a new wash step to the sequence: Click on a well in the plate view and drag it to the sequence.
• To change the pressure or duration of a wash step: First select the step to modify, then click on the property to edit.
• To change the position of a step in the sequence: Click on the dotted area of a step and drag it to the new position.

Click on the button to start the wash cycle.

Restore Pos remembers the position of the probe before starting the wash cycle and moves the probe back to this position once the wash cycle finishes.

Result history

All results (such as screenshots and probe images) are stored in the database.

Clicking a preview icon or thumbnail will open an enlarged view with the full details of the result. This will also provide the option to export the results to a file.

The result history offers different view modes, which can be chosen at the bottom of the result history:

Single column

Displays the result previews in a compact single column next to the tool bar.

Extended column

Displays the result previews in a single column as above, but with a few informative details:

1. An ID of the result in the database
2. The date and time (in minutes) when the result was acquired
3. The time since the first result was acquired

Multiple columns

Displays the result previews in multiple columns, such that more results are visible compared to single column mode. In this mode, you can export all results at the same time.

Accessible Plate Area

By activating this tool, the video feed will show the bounds of the accessible plate area. Inaccessible areas are indicated by crossing red lines.

This tool is activated automatically when using the preview functionality in the 3D printing workflow.

System Information

At the bottom of the application window, system status information is displayed.

• Currently applied pressure
• Current axes position as an (x ,y ,z) tuple. Note that erroneous values might be reported while an initialization procedure takes place.
• Currently measured ADC values and the resulting cantilever deflection. The deflection value might differ from the one shown in the oscilloscope because here the underlying ADC values are not normalized.
• A warning if the intensity of the cantilever’s deflection value drops below a certain threshold. This might be caused by disturbances within the laser path which implies a disabled force control mechanism. The warning is only supported after the laser was aligned and its signal maximized.
• Whether a probe is mounted
• Currently used sensitivity and stiffness values
• A control to digitally magnify the video feed
• The exchange camera button to switch between the top and bottom view camera video:

Result export

Results of the current experiment can be exported from the result history in various ways:

• A single result can be exported from the detailed result view (after clicking on a result preview in any of the views)
• All results can be exported from the multi-column view

Click on the export button to present the following dialog box showing the export options and file path:

Click on the export directory to change the directory where the result data is saved. If this path is too long to display, it will be truncated - hover the mouse over the path to see the full one.

The default name of the exported files includes the result type and the unique database ID of the result in the name.

Finally, the export option dialog allows the user to select which results are exported and offers a few export options depending on the result type.

Dragging

Some tools are overlaid over the video feed. These can be moved by dragging the black handle bar on their right side.

Cancellation

Buttons that start an operation usually also terminate it on a subsequent click. This is indicated by a red cross which overlays the button when cancellation is possible.

An example with the approach button:

State	Description
	An operation is ready to be started
	The operation is running - press again to abort
	The operation is currently not available; either it is being stopped or another operation is running

Arrow navigation

In various situations (e.g. during Laser Alignment) fine grained control over a movement is necessary. In this case an arrow navigation tool as depicted will be available.

Clicking on the arrow keys will perform a movement in the corresponding direction by the distance indicated on the right by step size. The step size can be changed with the + and - buttons.

Clicking on the button will reset all axes to their initial position (this button may not be available in all situations).

Hint: Keyboard navigation

Instead of clicking the buttons the arrow keys (←, →, ↑, ↓) on the keyboard can be used for navigation, the + and - keys on the keypad for step size control.

Number editor

Clicking on an editable number in the software brings up the number editor. Both the value and the unit of the current number can be changed. They only become effective once the number is applied.

The number editor validates the input and gives feedback if, for example, the unit is not supported or the value is out of range.

Hint

You may use u instead of µ (e.g. um = µm).

If the slider icon is present, clicking on it shows a slider instead of buttons to set the desired value.

Hint: Scientific notation

The number editor also supports scientific notation. E.g. one can write 1e-6 instead of 0.000001.

Stage movement

Standard dragging

While looking at a sample, clicking and maintaining pressure on the mouse’s left button will trigger a dragging process. At that point, the cursor will change to a grabbing hand. The standard dragging is meant to be intuitive: click on an object on the sample and drag it to the center of the screen, for instance.

Infinity movement

While the standard drag is the ideal way to carry out small movements, the need to travel longer distances within a well might arise. To this end, use the so called “infinity” drag. Clicking and holding the mouse’s left button while hovering around the edge of the screen and will trigger a constant movement. The switch between standard dragging and “infinity” movement is seamless.

A screenshot of an “infinity” movement indicated by the white arrow over a transparent border.

Boundaries

To prevent the probe hitting a plate, a boundary checker is implemented for each plate type. As a result, a red transparent border appears whenever a boundary is reached while moving the stage, be it when dragging or in “infinity movement” mode.

A screenshot of a boundary warning: the red arrows show the forbidden directions while the red, transparent border indicates a boundary was reached.

Settings

Settings Name	Description	Persistent
Approach Setpoint	The probe deflection value at which the approach will be stopped. Typically around 10 mV or 30 nN. Higher values are sometimes necessary if noise is encountered due to floating particles in the sample.	No
Approach Speed	How fast the probe approaches the sample. Higher values can drastically reduce the time to approach, but also increase the risk of probe damage. We recommend values around 5 µm/s.	No
Retract Speed	The speed at which the probe is lifted away from the surface.	No
Illumination	The system has an integrated LED illumination. Set its value here.	Yes
Objective	Choose which objective is used. It is important to adjust this value manually whenever changing the objective. The magnification is an important information for CAPA to know the right scale.	No
Show Help Lines	Choose whether to show help lines (a crosshair and a square indicating the size of a 100 by 100 µm area) in the center of the video view.	Yes
Idle Pressure	The pressure that will be constantly applied by the pressure system in idle situations (preventing liquid from flowing through the cantilever in either direction). This value is automatically updated after probe filling.	Yes